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The cholinergic neurons during the PPN and LDT regulate each SNc and VTA neurons as a result of activating acetylcholine receptors in these neurons [8]. Combining optogenetic and Mind slice patch-clamp strategies, Xiao et al. [8] demonstrated that 5–ten s of optogenetic stimulation of cholinergic projections from the PPN and LDT evokes inward c
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